Link to the University of Pittsburgh Homepage
Link to the University Library System Homepage Link to the Contact Us Form

Photodynamic release of nitric oxide from nitrosothiols of glutathione and serum albumin studied by transient absorption and transient circular dichroism spectroscopy

Leecharoen, Rojana (2005) Photodynamic release of nitric oxide from nitrosothiols of glutathione and serum albumin studied by transient absorption and transient circular dichroism spectroscopy. Master's Thesis, University of Pittsburgh. (Unpublished)

[img]
Preview
PDF
Primary Text

Download (663kB) | Preview

Abstract

Nitrosation of the free sulfhydryl group in peptides and proteins leads to one of their important biological functions, as nitric oxide carriers. Nitric oxide, an important signaling free radical molecule, cannot travel to many of its important targets by itself due to its short half-life. The S-NO bond in S-nitrosopeptides or proteins, however, is both thermolytically and photolytically labile, leading to release of NO which can then attack other targets such as other amino acids and nucleotides. In this thesis, photodynamic activity of NO released from a model peptide (glutathione) and a model protein (bovine serum albumin) is studied utilizing various spectroscopic tools. The more simple linear transient absorption spectroscopy gives information on the time-scale of the photocleavage as well as that of the subsequence geminate recombination, while the more difficult transient circular dichroism will give more insightful into the conformation changes of the peptide and protein surrounding the chromophore and the direction at which the NO is being released which will enable us to explore possible targets of the photoreleased NO. In addition to experimental study, many theoretical calculations are performed to aid understanding of the results.


Share

Citation/Export:
Social Networking:
Share |

Details

Item Type: University of Pittsburgh ETD
Status: Unpublished
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Leecharoen, Rojanarojanal@yahoo.com
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairWalker, Gilbert Cgwalker@chem.utoronto.ca
Committee MemberWaldeck, David Hdave@pitt.eduDAVE
Committee MemberBeratan, David N
Date: 7 June 2005
Date Type: Completion
Defense Date: 4 February 1999
Approval Date: 7 June 2005
Submission Date: 17 April 2005
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Institution: University of Pittsburgh
Schools and Programs: Dietrich School of Arts and Sciences > Chemistry
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: Nitric oxide; photochemistry
Other ID: http://etd.library.pitt.edu/ETD/available/etd-04172005-194026/, etd-04172005-194026
Date Deposited: 10 Nov 2011 19:37
Last Modified: 15 Nov 2016 13:40
URI: http://d-scholarship.pitt.edu/id/eprint/7243

Metrics

Monthly Views for the past 3 years

Plum Analytics


Actions (login required)

View Item View Item