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Purifacation of Wild Type EcoRI Endonuclease and EcoRI Endonuclease RS187Crystal Growth of WT EcoRI Endonuclease-DNA comlex and EcoRI Endonuclease

Dai, XiaoHu (2007) Purifacation of Wild Type EcoRI Endonuclease and EcoRI Endonuclease RS187Crystal Growth of WT EcoRI Endonuclease-DNA comlex and EcoRI Endonuclease. Master's Thesis, University of Pittsburgh. (Unpublished)

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Abstract

EcoRI endonuclease is a very useful tool to study the structural mechanism of protein-DNA recognition. In this work, wild type EcoRI Endonuclease and EcoRI Endonuclease mutant RS187 were purified to high purity. Crystals of wild type EcoRI Endonuclease-DNA 13mer complex have been obtained with good size and shape. Some small crystals of EcoRI Endonuclease RS187-DNA 13mer complex were also grown.


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Details

Item Type: University of Pittsburgh ETD
Status: Unpublished
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Dai, XiaoHuxid7@pitt.eduXID7
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairRosenberg, Johnjmr@jmr3.xtal.pitt.eduROSENBRG
Committee MemberRule, Gordon
Committee MemberTang, PeiTangP@anes.upmc.eduPTANG
Date: 19 September 2007
Date Type: Completion
Defense Date: 30 May 2007
Approval Date: 19 September 2007
Submission Date: 11 June 2007
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Institution: University of Pittsburgh
Schools and Programs: Dietrich School of Arts and Sciences > Molecular Biophysics
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: DNA; EcoRI endonuclease
Other ID: http://etd.library.pitt.edu/ETD/available/etd-06112007-125605/, etd-06112007-125605
Date Deposited: 10 Nov 2011 19:46
Last Modified: 19 Dec 2016 14:36
URI: http://d-scholarship.pitt.edu/id/eprint/8073

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