Complete Genomic Sequence of the Virulent Salmonella Bacteriophage SP6.

Dobbins, Aleisha T and George, Matthew and Basham, Daryl A and Ford, Michael E and Houtz, Jennifer M and Pedulla, Marisa L and Lawrence, Jeffrey G and Hatfull, Graham F and Hendrix, Roger W (2004) Complete Genomic Sequence of the Virulent Salmonella Bacteriophage SP6. Journal of bacteriology, 186 (7). 1933 - 1944. ISSN 0021-9193

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Abstract

We report the complete genome sequence of enterobacteriophage SP6, which infects Salmonella enterica serovar Typhimurium. The genome contains 43,769 bp, including a 174-bp direct terminal repeat. The gene content and organization clearly place SP6 in the coliphage T7 group of phages, but there is approximately 5 kb at the right end of the genome that is not present in other members of the group, and the homologues of T7 genes 1.3 through 3 appear to have undergone an unusual reorganization. Sequence analysis identified 10 putative promoters for the SP6-encoded RNA polymerase and seven putative rho-independent terminators. The terminator following the gene encoding the major capsid subunit has a termination efficiency of about 50% with the SP6-encoded RNA polymerase. Phylogenetic analysis of phages related to SP6 provided clear evidence for horizontal exchange of sequences in the ancestry of these phages and clearly demarcated exchange boundaries; one of the recombination joints lies within the coding region for a phage exonuclease. Bioinformatic analysis of the SP6 sequence strongly suggested that DNA replication occurs in large part through a bidirectional mechanism, possibly with circular intermediates.

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Details

Item Type:

Article

Status:

Published

Creators/Authors:

Creators	Email	Pitt Username
Dobbins, Aleisha T
George, Matthew
Basham, Daryl A
Ford, Michael E
Houtz, Jennifer M
Pedulla, Marisa L
Lawrence, Jeffrey G
Hatfull, Graham F	gfh@pitt.edu	GFH
Hendrix, Roger W

Date:

April 2004

Date Type:

Publication

Journal or Publication Title:

Journal of bacteriology

Volume:

186

Number:

Publisher:

American Society for Microbiology

Page Range:

1933 - 1944

DOI or Unique Handle:

10.1128/jb.186.7.1933-1944.2004

Schools and Programs:

Dietrich School of Arts and Sciences > Biological Sciences

Refereed:

Yes

ISSN:

0021-9193

Article Type:

Research Article

MeSH Headings:

Amino Acid Sequence; Base Sequence; DNA Replication; Gene Expression Regulation, Viral; Genome, Viral; Molecular Sequence Data; Open Reading Frames; Phylogeny; Promoter Regions, Genetic--genetics; Salmonella Phages--genetics; Salmonella typhimurium--virology; Sequence Analysis, DNA; Terminator Regions, Genetic--genetics; Viral Proteins--chemistry; Viral Proteins--genetics

Other ID:

NLM PMC374404

PubMed Central ID:

PMC374404

PubMed ID:

15028677

Date Deposited:

12 Nov 2012 15:49

Last Modified:

28 Sep 2022 13:11

URI:

http://d-scholarship.pitt.edu/id/eprint/16196