The sequence of the distal end of the E. coli ribosomal RNA rrnE operon indicates conserved features are shared by rrn operons

Liebke, H and Hatfull, G (1985) The sequence of the distal end of the E. coli ribosomal RNA rrnE operon indicates conserved features are shared by rrn operons. Nucleic Acids Research, 13 (15). 5515 - 5525. ISSN 0305-1048

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Abstract

The 1440 nucleotides of the distal region of the E.coli ribosocial RNA operon found on the λaroE transducing phage has been sequenced. We show that the λaroE hybrid rrn operon ends after asolitary 5S RHA gene with rrnE distal sequence. A single terminator structure of dyad symmetry followed by a run of six T's has been identified and compared to other sequenced rrn terminator hairpins. Immediately adjacent to the hairpin is a region of interrupted but conserved sequence that is shared by rrnE, rrnB and rraD. An open reading frame of 127 ammo acids abuts the terminator structure. Another open reading frame of 147 amino acids is found on the opposite strand several hundred nucleotides dovnstream. © 1985 IRL Press Limited.

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Details

Item Type:

Article

Status:

Published

Creators/Authors:

Creators	Email	Pitt Username	ORCID
Liebke, H
Hatfull, G	gfh@pitt.edu	GFH

Date:

12 August 1985

Date Type:

Publication

Journal or Publication Title:

Nucleic Acids Research

Volume:

Number:

Page Range:

5515 - 5525

DOI or Unique Handle:

10.1093/nar/13.15.5515

Schools and Programs:

Dietrich School of Arts and Sciences > Biological Sciences

Refereed:

Yes

ISSN:

0305-1048

MeSH Headings:

Bacteriophage lambda--genetics; Base Sequence; DNA Restriction Enzymes; DNA, Bacterial--genetics; Escherichia coli--genetics; Nucleic Acid Hybridization; Operon; RNA, Bacterial--genetics; RNA, Ribosomal--genetics

Other ID:

NLM PMC321887

PubMed Central ID:

PMC321887

PubMed ID:

2412207

Date Deposited:

23 Nov 2015 14:56

Last Modified:

02 Feb 2019 15:57

URI:

http://d-scholarship.pitt.edu/id/eprint/26380