Gokhale, A and Rwigema, JC and Epperly, MW and Glowacki, J and Wang, H and Wipf, P and Goff, JP and Dixon, T and Patrene, K and Greenberger, JS
(2010)
Small molecule GS-nitroxide ameliorates ionizing irradiation-induced delay in bone wound healing in a novel murine model.
In Vivo, 24 (4).
377 - 385.
ISSN 0258-851X
Abstract
We studied radioprotection and mitigation by mitochondrial-targeted Tempol (GS-nitroxide, JP4-039), in a mouse injury/irradiation model of combined injury (fracture/irradiation). Right hind legs of control C57BL/6NHsd female mice, mice pretreated with MnSOD-PL, JP4-039, or with amifostine were irradiated with single and fractionated doses of 0 to 20 Gy. Twenty-four hours later, unicortical holes were drilled into the tibiae of both hind legs; at intervals, tibias were excised, radiographed, and processed for histology. Bone wounds irradiated to 20 or 30 Gy showed delayed healing at 21 to 28 days. Treatment with JP4-039 MnSOD-PL or amifostine, before or after single fraction 20 Gy or during fractionated irradiation followed by drilling accelerated wound healing at days 21 and 28. Orthotopic 3LL tumors were not protected by JP4-039 or amifostine. In nonirradiated mice, pretreatment with JP4-039 accelerated bone wound healing. This test system should be useful for the development of new small molecule radioprotectors.
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Item Type: |
Article
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Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID  |
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Gokhale, A | | | | Rwigema, JC | | | | Epperly, MW | | | | Glowacki, J | | | | Wang, H | how8@pitt.edu | HOW8 | 0000-0003-0477-2908 | Wipf, P | pwipf@pitt.edu | PWIPF | | Goff, JP | | | | Dixon, T | | | | Patrene, K | | | | Greenberger, JS | joelg@pitt.edu | JOELG | |
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Date: |
1 January 2010 |
Date Type: |
Publication |
Journal or Publication Title: |
In Vivo |
Volume: |
24 |
Number: |
4 |
Page Range: |
377 - 385 |
Schools and Programs: |
Dietrich School of Arts and Sciences > Chemistry |
Refereed: |
Yes |
ISSN: |
0258-851X |
MeSH Headings: |
Animals; Cyclic N-Oxides--therapeutic use; Disease Models, Animal; Dose-Response Relationship, Radiation; Female; Hindlimb; Mice; Mice, Inbred C57BL; Radiation Injuries--drug therapy; Radiation Injuries--prevention & control; Radiation, Ionizing; Radiation-Protective Agents--therapeutic use; Spin Labels; Tibia--drug effects; Tibia--injuries; Tibia--radiography; Wound Healing--radiation effects |
Other ID: |
NLM NIHMS223059, NLM PMC2916688 |
PubMed Central ID: |
PMC2916688 |
PubMed ID: |
20668303 |
Date Deposited: |
28 May 2013 15:30 |
Last Modified: |
20 Sep 2022 13:11 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/18777 |
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