Clendenen, TV and Arslan, AA and Lokshin, AE and Idahl, A and Hallmans, G and Koenig, KL and Marrangoni, AM and Nolen, BM and Ohlson, N and Zeleniuch-Jacquotte, A and Lundin, E
(2010)
Temporal reliability of cytokines and growth factors in EDTA plasma.
BMC Research Notes, 3.
Abstract
Background. Cytokines are involved in the development of chronic diseases, including cancer. It is important to evaluate the temporal reproducibility of cytokines in plasma prior to conducting epidemiologic studies utilizing these markers. Findings. We assessed the temporal reliability of CRP, 22 cytokines and their soluble receptors (IL-1α, ILβ-1, IL-1RA, IL-2, sIL-2R, IL-4, IL-5, IL-6, sIL-6R, IL-7, IL-8, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, IL-17, TNFα, sTNF-R1, sTNF-R2, IFNα, IFNγ) and eight growth factors (GM-CSF, EGF, bFGF, G-CSF, HGF, VEGF, EGFR, ErbB2) in repeated EDTA plasma samples collected an average of two years apart from 18 healthy women (age range: 42-62) enrolled in a prospective cohort study. We also estimated the correlation between serum and plasma biomarker levels using 18 paired clinical samples from postmenopausal women (age range: 75-86). Twenty-six assays were able to detect their analytes in at least 70% of samples. Of those 26 assays, we observed moderate to high intra-class correlation coefficients (ICCs)(ranging from 0.53-0.89) for 22 assays, and low ICCs (0-0.47) for four assays. Serum and plasma levels were highly correlated (r > 0.6) for most markers, except for seven assays (r < 0.5). Conclusions. For 22 of the 31 biomarkers, a single plasma measurement is a reliable estimate of a woman's average level over a two-year period. © 2010 Clendenen et al; licensee BioMed Central Ltd.
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Item Type: |
Article
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Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID  |
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Clendenen, TV | | | | Arslan, AA | | | | Lokshin, AE | lokshina@pitt.edu | LOKSHINA | | Idahl, A | | | | Hallmans, G | | | | Koenig, KL | | | | Marrangoni, AM | | | | Nolen, BM | bmn9@pitt.edu | BMN9 | | Ohlson, N | | | | Zeleniuch-Jacquotte, A | | | | Lundin, E | | | |
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Centers: |
Other Centers, Institutes, Offices, or Units > Pittsburgh Cancer Institute |
Date: |
16 November 2010 |
Date Type: |
Publication |
Journal or Publication Title: |
BMC Research Notes |
Volume: |
3 |
DOI or Unique Handle: |
10.1186/1756-0500-3-302 |
Schools and Programs: |
School of Medicine > Medicine School of Medicine > Obstetrics, Gynecology, and Reproductive Sciences School of Medicine > Pathology |
Refereed: |
Yes |
Date Deposited: |
16 Nov 2016 16:06 |
Last Modified: |
04 Feb 2019 15:58 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/30212 |
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