Cicinelli, Ettore and Vitagliano, Amerigo and Loizzi, Vera and De Ziegler, Dominique and Fanelli, Margherita and Bettocchi, Stefano and Nardelli, Claudia and Trojano, Giuseppe and Cicinelli, Rossana and Minervini, Crescenzio Francesco and Leronni, Daniela and Viggiano, Luigi
(2021)
Altered Gene Expression Encoding Cytochines, Grow Factors and Cell Cycle Regulators in the Endometrium of Women with Chronic Endometritis.
Diagnostics, 11 (3).
p. 471.
ISSN 2075-4418
Abstract
To evaluate the expression of genes encoding cytokines, grow factors and cell cycle regulators in the proliferative endometrium of women with chronic endometritis (CE) compared to controls. We performed a case-control study on seven women with CE as diagnosed by hysteroscopy and histology (Cases) compared to six women without CE (Controls). All women underwent diagnostic hysteroscopy plus endometrial biopsy during the mid-proliferative phase of the menstrual cycle. Endometrial samples were divided into two different aliquots for histological and molecular analyses. The endometrial expression profile of 16 genes encoding proteins involved in the inflammatory process, proliferation and cell cycle regulation/apoptosis was assessed by using high-throughput qPCR. Study endpoints were between-group differences in the expression of VEGF A, VEGF B, VEGF C, EGF, TNF, TGF B1, IFNG, TP73, TP73L, BAXva, CDC2, CDC2va, CCND3, CCNB1, BAX and IL12. RESULTS: VEGF A, VEGF B, VEGF C, EGF, TNF, TGF B1, IFNG, TP73, TP73L, BAXva, CDC2, CDC2va, CCND3, CCNB1 were significantly overexpressed in women with CE compared to controls, while BAX and IL12 had similar expression between groups. In women with CE, we found an altered endometrial expression of genes involved in inflammatory, cell proliferation, and apoptosis processes. The dominance of proliferative and anti-apoptotic activity in CE may potentially promote the development of polyps and hyperplastic lesions.
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Details
| Item Type: |
Article
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| Status: |
Published |
| Creators/Authors: |
| Creators | Email | Pitt Username | ORCID  |
|---|
| Cicinelli, Ettore | | | | | Vitagliano, Amerigo | | | | | Loizzi, Vera | | | | | De Ziegler, Dominique | | | | | Fanelli, Margherita | | | | | Bettocchi, Stefano | | | | | Nardelli, Claudia | | | | | Trojano, Giuseppe | | | | | Cicinelli, Rossana | | | | | Minervini, Crescenzio Francesco | | | | | Leronni, Daniela | dal118@pitt.edu | dal118 | | | Viggiano, Luigi | | | |
|
| Date: |
8 March 2021 |
| Date Type: |
Publication |
| Journal or Publication Title: |
Diagnostics |
| Volume: |
11 |
| Number: |
3 |
| Publisher: |
MDPI AG |
| Page Range: |
p. 471 |
| DOI or Unique Handle: |
10.3390/diagnostics11030471 |
| Schools and Programs: |
School of Medicine > Neurological Surgery |
| Refereed: |
Yes |
| Uncontrolled Keywords: |
endometrium, chronic endometritis, real time RT-PCR, gene expression, growth factors |
| ISSN: |
2075-4418 |
| Official URL: |
http://dx.doi.org/10.3390/diagnostics11030471 |
| Article Type: |
Research Article |
| Date Deposited: |
21 Jul 2021 20:06 |
| Last Modified: |
21 Jul 2021 20:06 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/41442 |
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