Bozym, RA and Morosky, SA and Kim, KS and Cherry, S and Coyne, CB
(2010)
Release of intracellular calcium stores facilitates coxsackievirus entry into polarized endothelial cells.
PLoS Pathogens, 6 (10).
ISSN 1553-7366
Abstract
Group B coxsackieviruses (CVB) are associated with viral-induced heart disease and are among the leading causes of aseptic meningitis worldwide. Here we show that CVB entry into polarized brain microvasculature and aortic endothelial cells triggers a depletion of intracellular calcium stores initiated through viral attachment to the apical attachment factor decayaccelerating factor. Calcium release was dependent upon a signaling cascade that required the activity of the Src family of tyrosine kinases, phospholipase C, and the inositol 1,4,5-trisphosphate receptor isoform 3. CVB-mediated calcium release was required for the activation of calpain-2, a calcium-dependent cysteine protease, which controlled the vesicular trafficking of internalized CVB particles. These data point to a specific role for calcium signaling in CVB entry into polarized endothelial monolayers and highlight the unique signaling mechanisms used by these viruses to cross endothelial barriers. © 2010 Bozym et al.
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Details
Item Type: |
Article
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Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID  |
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Bozym, RA | | | | Morosky, SA | stm39@pitt.edu | STM39 | | Kim, KS | | | | Cherry, S | | | | Coyne, CB | | | |
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Contributors: |
Contribution | Contributors Name | Email | Pitt Username | ORCID  |
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Editor | Hope, Thomas J. | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED |
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Date: |
1 October 2010 |
Date Type: |
Publication |
Journal or Publication Title: |
PLoS Pathogens |
Volume: |
6 |
Number: |
10 |
DOI or Unique Handle: |
10.1371/journal.ppat.1001135 |
Refereed: |
Yes |
ISSN: |
1553-7366 |
MeSH Headings: |
Calcium--metabolism; Calcium Signaling--physiology; Calpain--metabolism; Calpain--physiology; Cell Polarity--physiology; Cells, Cultured; Coxsackievirus Infections--metabolism; Endothelial Cells--metabolism; Endothelial Cells--physiology; Endothelial Cells--virology; Endothelium, Vascular--metabolism; Endothelium, Vascular--physiology; Endothelium, Vascular--virology; Enterovirus--physiology; Epithelium--metabolism; Epithelium--physiology; Epithelium--virology; Humans; Inositol 1,4,5-Trisphosphate Receptors--metabolism; Inositol 1,4,5-Trisphosphate Receptors--physiology; Intracellular Space--metabolism; Phospholipase C gamma--metabolism; Phospholipase C gamma--physiology; Virus Internalization |
Other ID: |
NLM PMC2951373 |
PubMed Central ID: |
PMC2951373 |
PubMed ID: |
20949071 |
Date Deposited: |
22 Aug 2012 21:50 |
Last Modified: |
23 Jan 2019 01:55 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/13556 |
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